P-214: Parental Contribution of HLA-G*0106 and G*0105N to Repeated Implantation Failure

Background: HLA-G, as a tolerogenic molecule, expressed in cytotrophoblast cells and playing an important role in the suppression of the immune response in maternal decidua. Today Interaction of the extracellular domains of HLA-G protein with cell receptors of immune system, including CD8, LILRB1, LILRB2 and killer cell immunoglobulin-like receptor KIR2DL4, was well known. Materials and Methods: To investigation of association between HLA-G *0106 and G*0105N with repeated implantation failure (RIF), we used polymerase chain reaction (PCR) - sequencing technique for exon 2, 3, 4 and intron 2 of HLA-G gene in 100 couples with two or more failed assisted reproductive technology (ART) in their history and 50 couples with normal fertility and had one or more child that referred to Royan institute of Iran. Results: The obtained results indicate that HLA-G *0106 and G*0105N alleles were significantly higher in the patients group compared to controls group (G *0106: p=0.002; OR=3.98; 95% CI = 1.54-10.28 and G*0105N: p=0.01; OR=0). Conclusion: Although HLA-G gene polymorphisms do not clearly affect on the risk for implantation failure in most couples who undergone ART, but allelic variation, specially, in exon3, exon4 of HLA-G gene can led to ART failure in human embryos, and also exist of *0106 and G*0105N alleles in the parents could be a prognostic factor for implantation failure.